2-way analysis of variance (anova) and holm-sidak multiple comparisons tests  (GraphPad Software Inc)

Journal: Translational Vision Science & Technology

Article Title: Treatment Efficacy of a Dual Release of Aflibercept and Dexamethasone From a Single Hydrogel Drug Delivery System in a Rodent Model

doi: 10.1167/tvst.14.6.31

Figure Lengend Snippet: Percent changes in CNV lesion area relative to week 0 for all treatment groups at each time point. Experiment groups included: No Treatment ( black ; n = 30 lesions), Blank-DDS ( blue ; n = 28 lesions), Bolus AFL ( pink ; n = 36 lesions), AFL-DDS ( green ; n = 35 lesions), DEX-DDS ( dark purple ; n = 35 lesions), and Combo-DDS ( light purple ; n = 32 lesions). Statistically significant differences were determined between groups using Holm-Sidak multiple comparisons tests after repeated-measures 2-way ANOVA. Statistical differences of P < 0.05 is represented by *, P < 0.01 is represented by **, P < 0.001 is represented by ***, and P < 0.0001 by ****.

Article Snippet: Statistical analysis will be performed with 2-way analysis of variance (ANOVA) and Holm-Sidak multiple comparisons tests (GraphPad Prism 9).

Techniques:

Journal: Translational Vision Science & Technology

Article Title: Treatment Efficacy of a Dual Release of Aflibercept and Dexamethasone From a Single Hydrogel Drug Delivery System in a Rodent Model

doi: 10.1167/tvst.14.6.31

Figure Lengend Snippet: Histological evaluation of CNV lesions. ( a ) Representative histology images of CNV lesions from the No Treatment group ( black ), the Blank-DDS group ( blue ), the Bolus AFL group ( pink ), the AFL-DDS group ( green ), the DEX-DDS group ( dark purple ), and the Combo-DDS group ( light purple ). ( b ) Representative images for No Treatment. ( c ) Representative image for Combo-DDS. Statistically significant differences between groups were determined using Holm-Sidak multiple comparisons tests after repeated-measures 1-way ANOVA. Statistical differences of P <0.05 is represented by *.

Article Snippet: Statistical analysis will be performed with 2-way analysis of variance (ANOVA) and Holm-Sidak multiple comparisons tests (GraphPad Prism 9).

Techniques:

Journal: Infection and Immunity

Article Title: Metabolic diversity of human macrophages: potential influence on Staphylococcus aureus intracellular survival

doi: 10.1128/iai.00474-23

Figure Lengend Snippet: S. aureus MprF is critical for intracellular survival in HMDMs and biofilm formation. HMDMs were exposed to S. aureus WT or ∆ mprF under ( A ) competition or ( B ) mono-infection conditions and lysed to quantify intracellular bacterial burden at the indicated time points using a gentamicin protection assay ( n = 4 from one experiment; * P < 0.05 and **** P < 0.0001; two-way ANOVA with Sidak’s multiple comparisons test). ( C ) To determine the effect of intracellular survival on biofilm formation, HMDMs were stained with CellTracker Deep Red (red) and then infected with either GFP-expressing S. aureus WT pCM29 or ∆ mprF pCM29 (green). Z-stacks (1 µM) were acquired using confocal microscopy at 2- or 24-h post-infection and 3D rendering was performed.

Article Snippet: Significant differences were determined using a two-way analysis of variance (ANOVA) with Sidak’s multiple comparisons test, one-way ANOVA with Dunnett’s multiple comparisons test, or unpaired Student’s t -test using GraphPad Prism version 9.0.2.

Techniques: Infection, Staining, Expressing, Confocal Microscopy